Various geographical regions of the world (Mathew et al., 2006). coli exhibit a highĭegree of both phenotypic and genetic diversity and disseminate in Gastrointestinal tract (Lloyd et al., 2007). coli population that causes the disease outside the coli strains are classified into two main May cause diarrhea and other diseases outside the gastrointestinal Significant part of microbial flora of healthy population. coli are large group of bacteria mainly living in the intestine UTIs manifesting 90% urine positive cultures from UTI patients (Ronald,Į. coli is the most common pathogen being responsible for Numerous bacterial species such as Mycoplasma, Klebsiella,Įnterobacter, Pseudomonas, Proteus, Acinetobacter, Staphylococci, andĬitrobacter may contribute towards UTI (Hooton and Stamm, 1997). Urination, pain above the pubic bone and hematuria (Ronald et al.,Ģ001). Symptoms of UTI include burning pain during urination, frequent Urinary bladder, and urethra, respectively (Bien et al., 2012). The UTIs are classified according to the site of infection suchĪs pyelonephritis, cystitis and urethritis for infections of kidneys, Higher risk of UTI because of some anatomical reasons (Daniele et al.,Ģ011). Nosocomial infections are considered as a significant problem of healthĬare systems (Johansen et al., 2006). UTI being responsible for almost 40% of all Infections of the human body affecting 8.1 million individuals per annum The urinary tract infection (UTI) is one of the most common Urinary tract infection, Escherichia coli, 16S rRNA, phylogenetic Understanding about the epidemiology of this organism and may serve asīaseline information for future researchers in the days ahead. coli are prevalent in the clinical setting of Pakistan With ST-648 strain followed by ST-73 strain indicating genetic closeness ST-101 and ST-14 strains while seven isolates showed high similarity Eight isolates resembled closely and grouped with each of Isolates (n=16) had close relatedness with established uropathogenic Through Neighbor-Joining method revealed that majority of E. Phylogenetic treeĬonstructed on the basis of 613 bp fragment of 16S rRNA, using MEGA6.1 Sequences wereĪligned and edited using CodonCode Aligner. Isolates, partial 16S rRNA was amplified and sequenced. Isolated from patients followed by Pseudomonas sp. coli was the most frequent bacterium (n=51, 60.7%) Out of total 174 samples, 84 (48%) wereįound urine culture positive with CFU >104.Īmong them, E. The bacterial species were identified by standardīiochemical and molecular tests. Males) patients with clinical symptoms of UTI from Gujranwala andįaisalabad districts of Pakistan, were cultured for the isolation ofīacterial agents. The clean clutch midstream urine samples of 174 (78% females and 22% Responsible for UTIs and to investigate its genetic lineage in Pakistan. The aim of the present study was to determine the foremost pathogen Most UTIs are caused by Escherichia coli (E.Ĭoli) which exhibit a high degree of both phenotypic and geneticĭiversity and disseminated in various geographical regions of the world. UTI can occurĪnywhere along the urinary tract and may cause symptomatic infection toĬomplete renal failure. Infections that infect millions of people worldwide. Urinary Tract Infections (UTIs) are the second most common Muhammad Hidayat Rasool, Fareeda Tasneem, Tamoor Hamid, Asima Tayyeb and Retrieved from īyline: Naveed Shahzad, Bilal Aslam, Iqra Hussain, Muhammad Ijaz, APA style: Distribution and Phylogenetic Analysis of Bacterial Isolates from Urinary Tract Infection Patients of Pakistan.Distribution and Phylogenetic Analysis of Bacterial Isolates from Urinary Tract Infection Patients of Pakistan." Retrieved from MLA style: "Distribution and Phylogenetic Analysis of Bacterial Isolates from Urinary Tract Infection Patients of Pakistan." The Free Library.
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